Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoblotting

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all UBR4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBR4 Antibody

Western Blot: UBR4 Antibody [NBP1-28730]

Western Blot: UBR4 Antibody [NBP1-28730]

Western Blot: UBR4 Antibody [NBP1-28730] - Whole cell lysate from NIH3T3, TCMK-1, 4T1, and CT26.WT cells.
Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: UBR4 Antibody [NBP1-28730]

Immunoprecipitation: UBR4 Antibody [NBP1-28730]

Immunoprecipitation: UBR4 Antibody [NBP1-28730] - Detection of Human p600 on HeLa whole cell lysate using NBP28730. p600 was also immunoprecipitated by rabbit anti-p600 antibodies NBP1-28683 and NBP1-28724.

Applications for UBR4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBR4

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Alternate Names

600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein

Entrez Gene IDs

23352 (Human)

Gene Symbol

UBR4

UniProt

Q5T4S7 (Human)

Additional UBR4 Products

Product Documents for UBR4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBR4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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