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UBR5/EDD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1220

Novus Biologicals, part of Bio-Techne
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NB100-1220

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-LAIKTKNFGFV corresponding to C-Terminus according to NP_056986.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBR5/EDD Antibody

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Applications for UBR5/EDD Antibody

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Peptide ELISA

Detection limit 1:4000
Application Notes
IP: Anonymous customers have reported positive results on human breast cancer cells, mouse lung tissue, rat lung tissue, and rabbit heart tissue. WB: Anonymous customers have reported positive results on human breast cancer cells (MDA-MB-468), human HeLa cells, human glioblastoma (T98G cells), mouse fibroblasts (NIH 3T3), and rabbit heart tissue. IHC-P: Human testis shows nuclear staining in all germ cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBR5

This is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Long Name

Ubiquitin Protein Ligase E3 Component N-recognin 5

Alternate Names

DD5, EDD, EDD1, HYD

Entrez Gene IDs

51366 (Human); 70790 (Mouse); 117060 (Rat)

Gene Symbol

UBR5

Additional UBR5 Products

Product Documents for UBR5/EDD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBR5/EDD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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