UCH-L1/PGP9.5 Antibody (BH7)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58869

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BH7

Concentration

1 mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

Recombinant full length human UCHL1 purified from E. coli. [Swiss-Prot# P09936]

Localization

Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note: About 30% of total UCHL1 is associated with membranes in brain.

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (BH7)

Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of tissue lysates using mouse mAb to UCHL1, NB110-58869, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24kDa corresponds to the UCHL1 protein.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (NB110-58869) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869]

Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of rat hippocampal section stained with mouse mAb to UCHL1, NB110-58869, dilution 1:5,000 in green and costained with rabbit pAb to FOX/NeuN, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.
View 1 more images

Applications for UCH-L1/PGP9.5 Antibody (BH7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:2000

Immunohistochemistry Free-Floating

1:2000

Simple Western

1:20000

Western Blot

1:20000
Application Notes
This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western Blot, where a band can be seen at approximately 24 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 29545 (Rat)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (BH7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UCH-L1/PGP9.5 Antibody (BH7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.