UCH-L1/PGP9.5 Antibody (rUCHL1/8057) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24044
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rUCHL1/8057
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for UCH-L1/PGP9.5 Antibody (rUCHL1/8057) - Azide and BSA Free
Immunogen
Recombinant full-length human UCH-L1/PGP9.5 protein
Reactivity Notes
Shows a broad species reactivity.
Localization
Cytoplasm. Endoplasmic reticulum membrane.
Marker
pan-Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C.
Applications for UCH-L1/PGP9.5 Antibody (rUCHL1/8057) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: UCH-L1/PGP9.5
UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.
Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.
Long Name
Ubiquitin C-terminal Hydrolase L1
Alternate Names
PARK5, PGP9.5, UCHL1
Gene Symbol
UCHL1
Additional UCH-L1/PGP9.5 Products
Product Documents for UCH-L1/PGP9.5 Antibody (rUCHL1/8057) - Azide and BSA Free
Product Specific Notices for UCH-L1/PGP9.5 Antibody (rUCHL1/8057) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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