UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20279
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2a Kappa Clone # rUCHL1/8133
Concentration
0.2 mg/ml
Product Summary for UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
Immunogen
Recombinant full-length human UCH-L1/PGP9.5 protein
Reactivity Notes
Shows a broad species reactivity
Localization
Cytoplasm. Endoplasmic reticulum membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Positive Controls: Cerebellum
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
Formalin-fixed, paraffin-embedded human cerebellum stained with UCH-L1/PGP9.5 antibody (rUCHL1/8133). Inset: PBS instead of primary antibody; secondary only negative control.Applications for UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UCH-L1/PGP9.5
UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.
Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.
Long Name
Ubiquitin C-terminal Hydrolase L1
Alternate Names
PARK5, PGP9.5, UCHL1
Gene Symbol
UCHL1
Additional UCH-L1/PGP9.5 Products
Product Documents for UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
Product Specific Notices for UCH-L1/PGP9.5 Antibody (rUCHL1/8133)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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