UCH-L1/PGP9.5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-676

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Equine (100%), Porcine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human UCHL1 protein (between residues 200-220). [UniProt# P09936]

Localization

Cytoplasmic

Marker

pan-Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCH-L1/PGP9.5 Antibody

Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676]

Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676]

Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676]

Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676]

Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).
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Applications for UCH-L1/PGP9.5 Antibody

Application
Recommended Usage

Flow Cytometry

2-5 ug/million cells/ml

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

2.5 - 5.0 ug/mL

Immunohistochemistry-Frozen

reported in scientific literature (PMID 25041530)

Immunohistochemistry-Paraffin

2.5 - 5.0 ug/mL

Simple Western

1:2000

Western Blot

2 ug/mL
Application Notes
By Western blot a band at ~25 kDa is seen.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 3 using NB300-676 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine, 0.15 M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) was the first discovered de-ubiquitinating enzyme which implicates in processing of ubiquitin precursors and also of ubiquitinated proteins. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at C-terminal glycine of ubiquitin. It also binds to free monoubiquitin and prevents its degradation in lysosomes. Localized in cytoplasm and ER membrane as lipid-anchor, UCH-L1 expression is restricted to brain, peripheral nerves, endocrine tissues and gonads of both sexes etc. UCH-L1 deletion in mice leads to fatal neurodegenerative disorder known as gracile axonal dystrophy and it is down-regulated in brains from Parkinson as well as Alzheimer disease patients. Expression outside of neuro-endocrine tissues is found in various cancers including B-cell lymphoma, multiple myeloma, and lung cancer. In transgenic mouse model, UCH-L1 has been demonstrated as an oncogene that causes malignancies by boosting AKT signalling. Furthermore, UCH-L1 has been shown to interfere with ubiquitination of RAPTOR which is catalyzed by DDB1-Cul4 E3 ligase complex, leading to loss of mTORC1 integrity accompanied by a concurrent increase in mTORC2, likely due to increased availability of free mTOR.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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