UCH-L3 Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90878

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Rat

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UCH-L3 Antibodies »

Product Summary for UCH-L3 Antibody (007)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Rat UCH-L3 (Uniprot#: Q91Y78; Glu2-Ala230).

Specificity

Has cross-reactivity in ELISA with: Human UCHL3Mouse UCHL3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UCH-L3 Antibody (007)

Western Blot: UCH-L3 Antibody (007) [NBP2-90878]

Western Blot: UCH-L3 Antibody (007) [NBP2-90878]

Western Blot: UCH-L3 Antibody (007) [NBP2-90878] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:31 kDa.
Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878]

Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878]

Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878] - Immunochemical staining of rat UCHL3 in rat testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878]

Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878]

Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878] - rat UCHL3 was immunoprecipitated using: Lane A:0.5 mg k562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. 2 uL anti-rat UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-rat UCHL3 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

Applications for UCH-L3 Antibody (007)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L3

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for UCH-L3 Antibody (007)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UCH-L3 Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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