UCH-L3 Antibody (153)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90679
Recombinant Monoclonal Antibody
![Western Blot: UCH-L3 Antibody (153) [NBP2-90679]](https://resources.bio-techne.com/images/products/UCH-L3-Antibody-153-Western-Blot-NBP2-90679-img0004.jpg "Western Blot: UCH-L3 Antibody (153) [NBP2-90679]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 153
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for UCH-L3 Antibody (153)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCH-L3 (Uniprot#: Q9JKB1; Glu2-Ala230).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for UCH-L3 Antibody (153)
Western Blot: UCH-L3 Antibody (153) [NBP2-90679]
Western Blot: UCH-L3 Antibody (153) [NBP2-90679] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa. (We are unsure as to the identity of these extra bands.)![Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679]](https://resources.bio-techne.com/images/products/UCH-L3-Antibody-153-Immunoprecipitation-NBP2-90679-img0003.jpg "Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679]")
Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679]
Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679] - Mouse UCHL3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg 293T Whole Cell Lysate. 2 uL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse UCHL3 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:Applications for UCH-L3 Antibody (153)
Application
Recommended Usage
Immunoprecipitation
0.5-2 ul/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UCH-L3
Long Name
Ubiquitin C-terminal Hydrolase L3
Alternate Names
Ubiquitin Thioesterase L3, UCHL3
Gene Symbol
UCHL3
Additional UCH-L3 Products
Product Documents for UCH-L3 Antibody (153)
Product Specific Notices for UCH-L3 Antibody (153)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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