UCH-L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49711

Novus Biologicals, part of Bio-Techne
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NBP1-49711
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all UCH-L3 Antibodies »

Product Summary for UCH-L3 Antibody

Immunogen

The immunogen for this product maps to a region between residue 174 and 224 of human Ubiquitin Carboxyl-Terminal Esterase L3 using the numbering given in entry NP_005993.1 (GeneID 7347).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UCH-L3 Antibody

Western Blot: UCH-L3 Antibody [NBP1-49711]

Western Blot: UCH-L3 Antibody [NBP1-49711]

Western Blot: UCH-L3 Antibody [NBP1-49711] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody NBP1-49711 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody NBP1-49710, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-49711]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-49711]

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-49711] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- UCH-L3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for UCH-L3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UCH-L3

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Four distinct UCH activities from bovine thymus have been identified. All 4 are thiol proteases and have high-affinity binding sites for ubiquitin. UCHL3 protein is 54% identical to that of UCHL1.

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for UCH-L3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UCH-L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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