UCP1 Antibody (1345)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43551

Novus Biologicals, part of Bio-Techne
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NBP2-43551
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1345

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UCP1 Antibodies »

Product Summary for UCP1 Antibody (1345)

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Reactivity Notes

Rat (80%), Rabbit (86%), Sheep (83%), Bovine (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCP1 Antibody (1345)

Immunohistochemistry-Paraffin: UCP1 Antibody (1345) [NBP2-43551]

Immunohistochemistry-Paraffin: UCP1 Antibody (1345) [NBP2-43551]

Immunohistochemistry-Paraffin: UCP1 Antibody (1345) [NBP2-43551] - UCP1 antibody [GT1345] detects UCP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 antibody [GT1345] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
UCP1 Antibody (1345)

Western Blot: UCP1 Antibody (1345) [NBP2-43551] -

Western Blot: UCP1 Antibody (1345) [NBP2-43551] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody [GT1345] (NBP2-43551) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for UCP1 Antibody (1345)

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UCP1

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Gene Symbol

UCP1

Additional UCP1 Products

Product Documents for UCP1 Antibody (1345)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UCP1 Antibody (1345)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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