Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Vab12 P4B12*A12

Format

BSA Free

Concentration

1 mg/ml

View all UCP1 Antibodies »

Product Summary for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

Immunogen

Ovalbumin-conjugated synthetic peptide - (10 amino acids) derived from the middle polypeptide region of human UCP1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The clone number is listed as Vab12 P4B12*A12 in the scientific literature.

Scientific Data Images for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

Western Blot: UCP1 Antibody (Vab12 P4B12*A12)BSA Free [NBP2-50343]

Western Blot: UCP1 Antibody (Vab12 P4B12*A12)BSA Free [NBP2-50343]

Western Blot: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Analysis of Jurkat cell lysates and Anti-UCP1 clone Vab12 P4B12 A12.
Immunohistochemistry: UCP1 Antibody (Vab12 P4B12*A12) - BSA Free [NBP2-50343]

Immunohistochemistry: UCP1 Antibody (Vab12 P4B12*A12) - BSA Free [NBP2-50343]

Immunohistochemistry: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Staining of human breast cancer tissue shows cytoplasmic positivity.

Applications for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): WB- Jurkat whole cell lysate or overexpression cell lysate; IHC-formalin-fixed, paraffin-embedded human breast cancer.

The clone number is listed as Vab12 P4B12*A12 in the scientific literature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCP1

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Gene Symbol

UCP1

Additional UCP1 Products

Product Documents for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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