UCP2 Antibody (3F1B9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52582

Novus Biologicals, part of Bio-Techne
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NBP2-52582-0.025mg
NBP2-52582
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3F1B9

Format

BSA Free

Concentration

1 mg/ml

View all UCP2 Antibodies »

Product Summary for UCP2 Antibody (3F1B9) - BSA Free

Immunogen

Purified recombinant fragment of human UCP2 (AA: 1-309) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCP2 Antibody (3F1B9) - BSA Free

Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis using UCP2 mAb against human UCP2 (AA: 1-309) recombinant protein. (Expected MW is 36.1 kDa)
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Immunocytochemistry/ Immunofluorescence: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Immunocytochemistry/Immunofluorescence: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Flow Cytometry: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Flow Cytometry: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green) and negative control (red).
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Applications for UCP2 Antibody (3F1B9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCP2

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Long Name

Uncoupling Protein 2

Alternate Names

SLC25A8, UCPH

Gene Symbol

UCP2

Additional UCP2 Products

Product Documents for UCP2 Antibody (3F1B9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UCP2 Antibody (3F1B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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