UHRF1 Antibody (3B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00029128-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UHRF1 Antibodies »

Product Specifications

Immunogen

UHRF1 (NP_037414, 694 a.a. ~ 793 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WNEVLASLKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDRSFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR

Reactivity Notes

Human. Other species not tested.

Specificity

UHRF1 - ubiquitin-like, containing PHD and RING finger domains, 1 (3B12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for UHRF1 Antibody (3B12)

Western Blot: UHRF1 Antibody (3B12) [H00029128-M02]

Western Blot: UHRF1 Antibody (3B12) [H00029128-M02]

Western Blot: UHRF1 Antibody (3B12) [H00029128-M02] - UHRF1 monoclonal antibody (M02), clone 3B12 Analysis of UHRF1 expression in MCF-7.
Immunocytochemistry/ Immunofluorescence: UHRF1 Antibody (3B12) [H00029128-M02]

Immunocytochemistry/ Immunofluorescence: UHRF1 Antibody (3B12) [H00029128-M02]

Immunocytochemistry/Immunofluorescence: UHRF1 Antibody (3B12) [H00029128-M02] - Analysis of monoclonal antibody to UHRF1 on MCF-7 cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: UHRF1 Antibody (3B12) [H00029128-M02] - Detection limit for recombinant GST tagged UHRF1 is approximately 1ng/ml as a capture antibody.

Applications for UHRF1 Antibody (3B12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UHRF1

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Long Name

E3 ubiquitin-protein ligase UHRF1

Alternate Names

hNp95, hUHRF1, HuNp95, ICBP90, NP95, Nuclear protein 95, RNF106

Entrez Gene IDs

29128 (Human)

Gene Symbol

UHRF1

UniProt

NP_037414 (Human)

Additional UHRF1 Products

Product Documents for UHRF1 Antibody (3B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UHRF1 Antibody (3B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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