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UHRF1 Antibody (HL2287) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25732

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25732

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2287

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human UHRF1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UHRF1 Antibody (HL2287) - Azide and BSA Free

UHRF1 Antibody (HL2287) - Azide and BSA Free

Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -

Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
UHRF1 Antibody (HL2287) - Azide and BSA Free

Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -

Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
UHRF1 Antibody (HL2287) - Azide and BSA Free

Knockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -

Knockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for UHRF1 Antibody (HL2287) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UHRF1

UHRF1 encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Long Name

E3 ubiquitin-protein ligase UHRF1

Alternate Names

hNp95, hUHRF1, HuNp95, ICBP90, NP95, Nuclear protein 95, RNF106

Gene Symbol

UHRF1

Additional UHRF1 Products

Product Documents for UHRF1 Antibody (HL2287) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UHRF1 Antibody (HL2287) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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