UHRF1 Antibody (HL2287) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25732
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2287
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for UHRF1 Antibody (HL2287) - Azide and BSA Free
Immunogen
Recombinant fragment of human UHRF1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UHRF1 Antibody (HL2287) - Azide and BSA Free
Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -
Western Blot: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -
Immunohistochemistry-Paraffin: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. UHRF1 stained by UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minKnockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] -
Knockdown Validated: UHRF1 Antibody (HL2287) - Azide and BSA Free [NBP3-25732] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (NBP3-25732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for UHRF1 Antibody (HL2287) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UHRF1
Long Name
E3 ubiquitin-protein ligase UHRF1
Alternate Names
hNp95, hUHRF1, HuNp95, ICBP90, NP95, Nuclear protein 95, RNF106
Gene Symbol
UHRF1
Additional UHRF1 Products
Product Documents for UHRF1 Antibody (HL2287) - Azide and BSA Free
Product Specific Notices for UHRF1 Antibody (HL2287) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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