ULK1 [p Ser556] Antibody (JE59-28)

Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814]

Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814] - Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (NBP3-32814) at 1/1,000 dilution.

Lane 1: MCF7 whole cell lysate
Lane 2: MCF7 treated with 0.5uM Oligomycin for 30 minutes whole cell lysate
Lane 3: MCF7 treated with pp for 1 hour whole cell lysate
Lane 4: MCF7 treated with 0.5uM Oligomycin for 30 minutes, then treated with pp for 1 hour whole cell lysate

Lysates/proteins at 14 ug/Lane.

Predicted band size: 113 kDa
Observed band size: 150 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32814) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.