Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation (Negative), Knockdown Validated, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all UNG Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 263 and 313 of human Uracil-DNA Glycosylase using the numbering given in entry NP_550433.1 (GeneID 7374).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UNG Antibody

Knockdown Validated: UNG Antibody [NBP1-49985]

Knockdown Validated: UNG Antibody [NBP1-49985]

Knockdown Validated: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for UNG Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-49985 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UNG

The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.

Long Name

Uracil N-Glycosylase

Alternate Names

DGU, UDG

Gene Symbol

UNG

Additional UNG Products

Product Documents for UNG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UNG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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