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uPAR Antibody (HL2169) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25734

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25734

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2169

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human uPAR.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for uPAR Antibody (HL2169) - Azide and BSA Free

uPAR Antibody (HL2169) - Azide and BSA Free

Immunohistochemistry-Paraffin: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] -

Immunohistochemistry-Paraffin: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] - uPAR antibody [HL2169] detects uPAR protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. uPAR stained by uPAR antibody [HL2169] (NBP3-25734) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
uPAR Antibody (HL2169) - Azide and BSA Free

Knockout Validated: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] -

Knockout Validated: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] - Wild-type (WT) and PLAUR knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with uPAR antibody [HL2169] (NBP3-25734) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
uPAR Antibody (HL2169) - Azide and BSA Free

Western Blot: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] -

Western Blot: uPAR Antibody (HL2169) - Azide and BSA Free [NBP3-25734] - Untreated (-) and treated (+) U937 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with uPAR antibody [HL2169] (NBP3-25734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for uPAR Antibody (HL2169) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: uPAR

uPAR (Urokinase plasminogen activator receptor) is a glycoprotein I anchored surface receptor specific for urokinase plasminogen activator (uPA). Upon binding to uPAR, uPA converts the surface bound, large serum beta globulin plasminogen to plasmin. Plasmin, which is also designated fibrinolysin, is a trypsin-like enzyme that acts on Arg-Lys bonds and induces pericellular proteolysis in fibrin and fibrinogen, and thereby contributes to the systematic activation of the coagulation cascade. uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells and are required for tumor invasion and metastasis.

Long Name

Urokinase-type Plasminogen Activator Receptor

Alternate Names

PLAUR

Gene Symbol

PLAUR

Additional uPAR Products

Product Documents for uPAR Antibody (HL2169) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for uPAR Antibody (HL2169) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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