UPF3A Antibody (2E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00065110-M06

Novus Biologicals, part of Bio-Techne
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H00065110-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UPF3A Antibodies »

Product Specifications

Immunogen

UPF3A (AAH23569.1, 274 a.a. ~ 374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TGGGKQESCAPGAVVKARPMEGSLEEPQETSHSGSDKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPERLSRRSEDEQRWGKGPGQDRGKKGSQDSGAP

Specificity

UPF3A - UPF3 regulator of nonsense transcripts homolog A (yeast) (2E2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UPF3A Antibody (2E2)

Western Blot: UPF3A Antibody (2E2) [H00065110-M06]

Western Blot: UPF3A Antibody (2E2) [H00065110-M06]

Western Blot: UPF3A Antibody (2E2) [H00065110-M06] - Analysis of UPF3A expression in transfected 293T cell line by UPF3A monoclonal antibody (M06), clone 2E2. Lane 1: UPF3A transfected lysatE (51 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: UPF3A Antibody (2E2) [H00065110-M06]

Immunohistochemistry-Paraffin: UPF3A Antibody (2E2) [H00065110-M06]

Immunohistochemistry-Paraffin: UPF3A Antibody (2E2) [H00065110-M06] - Analysis of monoclonal antibody to UPF3A on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Sandwich ELISA: UPF3A Antibody (2E2) [H00065110-M06] - Detection limit for recombinant GST tagged UPF3A is 0.03 ng/ml as a capture antibody.

Applications for UPF3A Antibody (2E2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UPF3A

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

HUPF3A, Nonsense mRNA reducing factor 3A, regulator of nonsense transcripts 3A, RENT3AUp-frameshift suppressor 3 homolog A, UPF3 regulator of nonsense transcripts homolog A (yeast), UPF3hUpf3

Entrez Gene IDs

65110 (Human)

Gene Symbol

UPF3A

UniProt

AAH23569 (Human)

Additional UPF3A Products

Product Documents for UPF3A Antibody (2E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UPF3A Antibody (2E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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