Uroplakin III Antibody (PD00-09)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33086
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PD00-09
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Uroplakin III aa 250 to the C-terminus. (Uniprot: O75631)
Localization
Endoplasmic reticulum membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Uroplakin III Antibody (PD00-09)
Immunohistochemistry: Uroplakin III Antibody (PD00-09) [NBP3-33086]
Immunohistochemistry: Uroplakin III Antibody (PD00-09) [NBP3-33086] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Uroplakin III antibody (NBP3-33086) at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33086) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: Uroplakin III Antibody (PD00-09) [NBP3-33086]
Flow Cytometry: Uroplakin III Antibody (PD00-09) [NBP3-33086] - Flow cytometric analysis of Hela cells labeling Uroplakin III.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33086, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Applications for Uroplakin III Antibody (PD00-09)
Application
Recommended Usage
Flow Cytometry
1:500-1:1000
Immunohistochemistry-Paraffin
1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Uroplakin III
Alternate Names
MGC119178, UP3a, UPIII, UPIIIA, UPK3, UPK3A, uroplakin 3, uroplakin 3A, Uroplakin III, uroplakin3a, uroplakin-3a
Gene Symbol
UPK3A
Additional Uroplakin III Products
Product Documents for Uroplakin III Antibody (PD00-09)
Product Specific Notices for Uroplakin III Antibody (PD00-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...