USF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38290

Novus Biologicals, part of Bio-Techne
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NBP2-38290
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all USF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USF1 Antibody

Western Blot: USF1 Antibody [NBP2-38290]

Western Blot: USF1 Antibody [NBP2-38290]

Western Blot: USF1 Antibody [NBP2-38290] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: USF1 Antibody [NBP2-38290]

Immunocytochemistry/ Immunofluorescence: USF1 Antibody [NBP2-38290]

Immunocytochemistry/Immunofluorescence: USF1 Antibody [NBP2-38290] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: USF1 Antibody [NBP2-38290]

Immunohistochemistry-Paraffin: USF1 Antibody [NBP2-38290]

Immunohistochemistry-Paraffin: USF1 Antibody [NBP2-38290] - Staining of human tonsil shows strong nuclear positivity.

Applications for USF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USF1

USF1 encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

BHLHB11, bHLHb11FCHL1, Class B basic helix-loop-helix protein 11, FCHL, HYPLIP1, Major late transcription factor 1, MLTF, MLTFI, UEF, upstream stimulatory factor 1, upstream transcription factor 1, USF

Gene Symbol

USF1

UniProt

P22415 (Human)

Additional USF1 Products

Product Documents for USF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for USF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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