USP22 Antibody (18H6)

Western Blot: USP22 Antibody (18H6) [NBP3-26261] -

Western Blot: USP22 Antibody (18H6) [NBP3-26261] - Positive Western Blot detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate.
All lanes: USP22 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 60, 59 kDa
Observed band size: 55-72 kDa

Immunocytochemistry/Immunofluorescence: USP22 Antibody (18H6) [NBP3-26261] -

Immunocytochemistry/Immunofluorescence: USP22 Antibody (18H6) [NBP3-26261] - Staining of Hela cell with USP22 Antibody (18H6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 507-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: USP22 Antibody (18H6) [NBP3-26261] -

Flow Cytometry: USP22 Antibody (18H6) [NBP3-26261] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18H6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.