Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all USP24 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2410 and the C-terminus (residue 2460) of human Ubiquitin Specific Peptidase 24 using the numbering given in entry XP_371254.5 (GeneID 23358).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

294 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP24 Antibody

Western Blot: USP24 Antibody [NB100-40830]

Western Blot: USP24 Antibody [NB100-40830]

Western Blot: USP24 Antibody [NB100-40830] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP24 antibody used for WB at 0.1 ug/ml. Detection: hemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: USP24 Antibody [NB100-40830]

Immunoprecipitation: USP24 Antibody [NB100-40830]

Immunoprecipitation: USP24 Antibody [NB100-40830] - Detection of human USP24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP24 antibody NB100-40830 (lot 2) used for IP at 3 ug per reaction. USP24 was also immunoprecipitated by a previous lot of this antibody (lot 1) and a rabbit anti-USP24 antibody from Company B. For blotting immunoprecipitated USP24, NB100-40830 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for USP24 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP24

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 24

Alternate Names

Deubiquitinating enzyme 24, EC 3.4.19.12, FLJ31309, KIAA1057ubiquitin carboxyl-terminal hydrolase 24, ubiquitin specific peptidase 24, ubiquitin specific protease 24, ubiquitin thioesterase 24, Ubiquitin thiolesterase 24, ubiquitin-specific processing protease 24, Ubiquitin-specific-processing protease 24

Entrez Gene IDs

23358 (Human)

Gene Symbol

USP24

UniProt

Q9UPU5 (Human)

Additional USP24 Products

Product Documents for USP24 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP24 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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