USP28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-96911

Novus Biologicals, part of Bio-Techne
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NB100-96911
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all USP28 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SVELKHYIQEDN corresponding to internal region according to NP_065937.1.

Epitope

SVELKHYIQEDN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for USP28 Antibody

Immunohistochemistry-Paraffin: USP28 Antibody [NB100-96911]

Immunohistochemistry-Paraffin: USP28 Antibody [NB100-96911]

Immunohistochemistry-Paraffin: USP28 Antibody [NB100-96911] - 3.8ug/ml staining of Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for USP28 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.2 ug/ml
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band and 50 kDa band in human bone marrow lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122 kDa band according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human testis shows acrosomal staining in spermatids.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP28

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Long Name

Ubiquitin Specific Protease 28

Alternate Names

Deubiquitinating enzyme 28, EC 3.1.2.15, EC 3.4.19.12, KIAA1515ubiquitin carboxyl-terminal hydrolase 28, ubiquitin specific peptidase 28, ubiquitin specific protease 28, ubiquitin thioesterase 28, Ubiquitin thiolesterase 28, Ubiquitin-specific-processing protease 28

Entrez Gene IDs

57646 (Human)

Gene Symbol

USP28

UniProt

NP_065937.1 (Human)

Additional USP28 Products

Product Documents for USP28 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for USP28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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