Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all USP3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 471 and 521 of human ubiquitin specific protease 3 using the numbering given in entry AAD42992.1 (GeneID 9960).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP3 Antibody

Immunohistochemistry-Paraffin: USP3 Antibody [NB100-77284]

Immunohistochemistry-Paraffin: USP3 Antibody [NB100-77284]

Immunohistochemistry-Paraffin: USP3 Antibody [NB100-77284] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: USP3 Antibody [NB100-77284]

Immunoprecipitation: USP3 Antibody [NB100-77284]

Immunoprecipitation: USP3 Antibody [NB100-77284] - Detection of Human USP3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP3 antibody NB100-77284used for IP at 3 mcg/mg lysate. USP3 was also immunoprecipitated by rabbit anti-USP3 antibody NB100-77283, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for USP3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP3

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B.

Long Name

Ubiquitin carboxyl-terminal hydrolase 3

Alternate Names

Deubiquitinating enzyme 3, EC 3.1.2.15, EC 3.4.19.12, MGC129878, MGC129879, SIH003, ubiquitin carboxyl-terminal hydrolase 3, ubiquitin specific peptidase 3, ubiquitin specific protease 3, ubiquitin thioesterase 3, Ubiquitin thiolesterase 3, Ubiquitin-specific-processing protease 3, UBP

Entrez Gene IDs

9960 (Human)

Gene Symbol

USP3

UniProt

AAD42992.1 (Human)

Additional USP3 Products

Product Documents for USP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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