Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all USP47 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP47 Antibody

Immunohistochemistry-Paraffin: USP47 Antibody [NB100-57487]

Immunohistochemistry-Paraffin: USP47 Antibody [NB100-57487]

Immunohistochemistry-Paraffin: USP47 Antibody [NB100-57487] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP47 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: USP47 Antibody [NB100-57487]

Immunoprecipitation: USP47 Antibody [NB100-57487]

Immunoprecipitation: USP47 Antibody [NB100-57487] - Detection of Human USP47 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody NB100-57487 used for IP at 3 mcg/mg lysate. USP47 was also immunoprecipitated by rabbit anti- USP47 antibody NB100-57486, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for USP47 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 mcg/mg
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP47

USP47 belongs to the peptidase C19 family and may be catalytically inactive although it seems to have kept all necessary active site residues. It is expressed in skeletal muscle, heart and testis and there are 3 named isoforms produced by alternative splicing.

Long Name

Ubiquitin Specific Protease 47

Alternate Names

TRFP

Entrez Gene IDs

55031 (Human)

Gene Symbol

USP47

UniProt

NP_060414.3 (Human)

Additional USP47 Products

Product Documents for USP47 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP47 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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