USP7 Antibody (03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06427

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all USP7 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human USP7 (Accession#: NP_003461.2; Lys208-Glu560).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for USP7 Antibody (03)

Immunocytochemistry/ Immunofluorescence: USP7 Antibody (03) [NBP3-06427]

Immunocytochemistry/ Immunofluorescence: USP7 Antibody (03) [NBP3-06427]

Immunocytochemistry/Immunofluorescence: USP7 Antibody (03) [NBP3-06427] - Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
Flow Cytometry: USP7 Antibody (03) [NBP3-06427]

Flow Cytometry: USP7 Antibody (03) [NBP3-06427]

Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for USP7 Antibody (03)

Application
Recommended Usage

ELISA

1:1000-1:2000

Flow Cytometry

1:25-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP7

HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Long Name

Ubiquitin Specific Protease 7

Alternate Names

HAUSP, TEF1

Gene Symbol

USP7

Additional USP7 Products

Product Documents for USP7 Antibody (03)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for USP7 Antibody (03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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