USP7 Antibody (7F10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26258
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7F10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human USP7 [UniProt Q93009]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for USP7 Antibody (7F10)
Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] -
Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] -
Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: USP7 Antibody (7F10) [NBP3-26258] -
Western Blot: USP7 Antibody (7F10) [NBP3-26258] - Positive Western Blot detected in: K562 whole cell lysate.All lanes: USP7 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 129, 127 kDa
Observed band size: 140 kDa
Applications for USP7 Antibody (7F10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: USP7
Long Name
Ubiquitin Specific Protease 7
Alternate Names
HAUSP, TEF1
Gene Symbol
USP7
Additional USP7 Products
Product Documents for USP7 Antibody (7F10)
Product Specific Notices for USP7 Antibody (7F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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