Uteroglobin/SCGB1A1 Antibody (JU34-03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75705

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU34-03

Concentration

1 mg/ml

View all Uteroglobin/SCGB1A1 Antibodies »

Product Summary for Uteroglobin/SCGB1A1 Antibody (JU34-03)

Immunogen

Synthetic peptide within Human Uteroglobin/SCGB1A1 aa 42-91 / 91. (SwissProt: P11684 Human; SwissProt: Q06318 Mouse; SwissProt: P17559 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Uteroglobin/SCGB1A1 Antibody (JU34-03)

Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Western blot analysis of Uteroglobin/SCGB1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: human lung tissue lysate Lane 3: A549 cell lysate
Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Immunocytochemistry/Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Staining SCGB1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]

Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SCGB1A1 antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for Uteroglobin/SCGB1A1 Antibody (JU34-03)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:1,000

Immunohistochemistry-Paraffin

1:1,000

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Uteroglobin/SCGB1A1

Uteroglobin, also known as secretoglobin family 1A member 1 (SCGB1A1), is a protein with anti-inflammatory and immunomodulatory properties. It is a member of the secretoglobin family, which includes small, secreted, disulfide-bridged dimeric proteins found only in mammals. Uteroglobin is a secretory protein induced by progesterone. It is present in the mucosal epithelia of nearly all organs and in many body fluids. The protein is also involved in anti-chemotactic, anti-allergic, anti-tumorigenic, and embryonic growth-stimulatory activities.

Alternate Names

Blastokinin, CC10, CC16, CCPBP, CCSP, Clara, PCB-BP, SCGB1A1, UGB

Gene Symbol

SCGB1A1

Additional Uteroglobin/SCGB1A1 Products

Product Documents for Uteroglobin/SCGB1A1 Antibody (JU34-03)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Uteroglobin/SCGB1A1 Antibody (JU34-03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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