V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81003
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6B11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide epitope in the CDR3 region of V alpha 24
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free
Flow (Cell Surface): V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003]
Flow (Cell Surface): V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003] - A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin. A co-stain was also performed using CD3 antibody NBP2-24867APC.Flow Cytometry: V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003]
Flow Cytometry: V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free [NBP2-81003] - Staining of normal human peripheral blood mononuclear cells with Anti-Human CD3 FITC and 1.0 ug of Mouse IgG1 K Isotype Control Purified (left) or 1.0 ug of Anti-Human V alpha 24 J alpha 18 TCR Purified (right) followed by F(ab')2 Anti-Mouse IgG PE . CD Image from the standard format of this antibody.Applications for V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 16867051)
Immunohistochemistry-Frozen
reported in multiple pieces of scientific literature
Application Notes
This 6B11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: V alpha 24 J alpha 18 TCR
Alternate Names
iNKT, iNKT cell, invariant natural killer T cell, invariant NK T cell, invariant NKT cell
Additional V alpha 24 J alpha 18 TCR Products
Product Documents for V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free
Product Specific Notices for V alpha 24 J alpha 18 TCR Antibody (6B11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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