V-type proton ATPase subunit F Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97190

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all V-type proton ATPase subunit F Antibodies »

Product Summary for V-type proton ATPase subunit F Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human V-type proton ATPase subunit F fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for V-type proton ATPase subunit F Antibody

Immunocytochemistry/ Immunofluorescence: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunocytochemistry/ Immunofluorescence: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunofluorescence staining of V-type proton ATPase subunit F in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human V-type proton ATPase subunit F polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunochemical staining of human V-type proton ATPase subunit F in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190]

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunochemical staining of human V-type proton ATPase subunit F in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for V-type proton ATPase subunit F Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: V-type proton ATPase subunit F

V-type proton ATPase subunit F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]

Alternate Names

ATP6S14adenosinetriphosphatase 14k chain, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F, H(+)-transporting two-sector ATPase, 14kD subunit, MGC117321, MGC126037, MGC126038, vacuolar ATP synthase subunit F, vacuolar proton pump F subunit, Vacuolar proton pump subunit F, VATFATPase, vacuolar, 14 kD, V-ATPase 14 kDa subunit, V-ATPase F subunit, V-ATPase subunit F, Vma7, V-type proton ATPase subunit F

Gene Symbol

ATP6V1F

Additional V-type proton ATPase subunit F Products

Product Documents for V-type proton ATPase subunit F Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for V-type proton ATPase subunit F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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