V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37825
![Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]](https://resources.bio-techne.com/images/products/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0003.jpg "Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # E10/V4RR
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
Immunogen
GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity
This product recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. It has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]
Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.![Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]](https://resources.bio-techne.com/images/products/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0004.jpg "Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]")
Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]
Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).Applications for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunofluorescence
1:500 - 1:2000
Immunoprecipitation
1:10 - 1:500
Western Blot
1:1000 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: V5 Epitope Tag
Alternate Names
GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5
Additional V5 Epitope Tag Products
Product Documents for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
Product Specific Notices for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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