V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52653
Recombinant Monoclonal Antibody.
![ELISA: V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free [NBP2-52653]](https://resources.bio-techne.com/images/products/V5-Epitope-Tag-Antibody-SV5-P-K-ELISA-NBP2-52653-img0001.jpg "ELISA: V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free [NBP2-52653]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Radioimmunoassay, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SV5-P-K
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Pk (V5) Epitope Tag (GKPIPNPLLGLDST).
Reactivity Notes
Paramyxovirus Simian Virus 5
Specificity
Recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the paramyxoVirus simian virus 5.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free
ELISA: V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free [NBP2-52653]
ELISA: V5 Epitope Tag Antibody (SV5-P-K) [NBP2-52653] - ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5ug/ml of Multitag protein. Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.Applications for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry-Frozen
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This is a chimeric rabbit antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: V5 Epitope Tag
Alternate Names
GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5
Additional V5 Epitope Tag Products
Product Documents for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free
Product Specific Notices for V5 Epitope Tag Antibody (SV5-P-K) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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