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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Chickens were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Chicken

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-379]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-379]

Western Blot: V5 Epitope Tag Antibody [NB600-379] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for V5 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:30000 (Primary) 1:100-1:500 (for coating plates)

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunohistochemistry

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000-1:30000
Application Notes
Use in IHC reported in scientific literature (PMID: 19021203)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for V5 Epitope Tag Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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