V5 Epitope Tag Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-380
![Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-380]](https://resources.bio-techne.com/images/products/V5-Epitope-Tag-Antibody-Western-Blot-NB600-380-img0005.jpg "Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-380]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Human, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Reactivity Notes
Human reactivity reported in multiple pieces of scientific literature.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for V5 Epitope Tag Antibody - BSA Free
Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-380]
Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for V5 Epitope Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
Primary-1:1000-1:30000; Coating plates-1:100-1:500
Immunocytochemistry/ Immunofluorescence
1:100-1:400
Immunoprecipitation
1 - 4ug/mg lysate
Western Blot
1:1000 - 1:30000
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 25767115).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: V5 Epitope Tag
Alternate Names
GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5
Additional V5 Epitope Tag Products
Product Documents for V5 Epitope Tag Antibody - BSA Free
Product Specific Notices for V5 Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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