VAMP-2 Antibody (GT6311)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13633

Novus Biologicals, part of Bio-Techne
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NBP3-13633
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (99%), Human (100%), Rhesus Macaque (100%), Xenopus (96%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT6311

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VAMP-2 Antibodies »

Product Summary for VAMP-2 Antibody (GT6311)

Immunogen

Full length human VAMP-2 Recombinant protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VAMP-2 Antibody (GT6311)

Western Blot: VAMP-2 Antibody (GT6311) [NBP3-13633]

Western Blot: VAMP-2 Antibody (GT6311) [NBP3-13633]

Western Blot: VAMP-2 Antibody (GT6311) [NBP3-13633] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 [GT6311] (NBP3-13633) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (GT6311) [NBP3-13633]

Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (GT6311) [NBP3-13633]

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody (GT6311) [NBP3-13633] - VAMP-2 antibody [GT6311] detects VAMP-2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP-2 stained by VAMP-2 antibody [GT6311] (NBP3-13633) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT6311) [NBP3-13633]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT6311) [NBP3-13633]

Immunohistochemistry-Paraffin: VAMP-2 Antibody (GT6311) [NBP3-13633] - VAMP-2 antibody [GT6311] detects VAMP-2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VAMP-2 stained by VAMP-2 antibody [GT6311] (NBP3-13633) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for VAMP-2 Antibody (GT6311)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP-2

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Long Name

Vesicle-Associated Membrane Protein 2

Alternate Names

SYB2, VAMP2

Gene Symbol

VAMP2

Additional VAMP-2 Products

Product Documents for VAMP-2 Antibody (GT6311)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAMP-2 Antibody (GT6311)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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