VAMP-7 Antibody (158.2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-07118

Novus Biologicals, part of Bio-Techne
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NBP1-07118
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 158.2

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all VAMP-7 Antibodies »

Product Specifications

Immunogen

TI-VAMP recombinant protein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Reacts specifically with the 25 kDa protein of human TI-VAMP protein (human, mouse, rat, canine)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VAMP-7 Antibody (158.2)

Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118]

Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118]

Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.
Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118]

Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118]

Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of MDCK cells (red). GFP-Tagged human Ti-VAMP (green) and merge (yellow).
Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118]

Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118]

Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of human spleen.

Applications for VAMP-7 Antibody (158.2)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:5-1:200

Immunohistochemistry-Paraffin

1:10 - 1:400

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Cell culture supernatant

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP-7

SYBL1 (Synaptobrevin-like 1) involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Found ubiquitously and is an unusual v-SNAREs molecule implicated in cellular exocytosis. The SYBL1 gene is found on the pseudoautosomal region (PAR) of X chromosome. It is a highly conserved gene and has been shown to be silenced on inactive X and Y chromosomes. In ICF syndrome, a human disease affecting DNA methylation, SYBL1 escapes from silencing and this correlates with the observed altered patterns of histone methylation and acetylation found. An association between some SYBL1 alleles and bipolar affective disorder (BPAD) has been observed. SUBUNIT: Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells.

Long Name

Vesicle-Associated Membrane Protein 7

Alternate Names

SYBL1, TI-VAMP, VAMP7

Entrez Gene IDs

20955 (Mouse); 85491 (Rat)

Gene Symbol

VAMP7

Additional VAMP-7 Products

Product Documents for VAMP-7 Antibody (158.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAMP-7 Antibody (158.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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