VAMP-8 Antibody (5H9S4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16471

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16471-100ul
NBP3-16471-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5H9S4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VAMP-8 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VAMP-8 (NP_003752.2). MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLICVIVFIIILFIVLFATGAFS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAMP-8 Antibody (5H9S4)

Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471] - Western blot analysis of extracts of various cell lines, using VAMP-8 antibody (NBP3-16471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Immunohistochemistry-Paraffin: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Immunohistochemistry-Paraffin: VAMP-8 Antibody (5H9S4) [NBP3-16471] - Immunohistochemistry of paraffin-embedded human appendix using VAMP-8 Rabbit mAb (NBP3-16471) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471]

Western Blot: VAMP-8 Antibody (5H9S4) [NBP3-16471] - Western blot analysis of extracts of various cell lines, using VAMP-8 antibody (NBP3-16471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for VAMP-8 Antibody (5H9S4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAMP-8

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.

Long Name

Vesicle-Associated Membrane Protein 8

Alternate Names

EDB, Endobrevin, VAMP8

Gene Symbol

VAMP8

Additional VAMP-8 Products

Product Documents for VAMP-8 Antibody (5H9S4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAMP-8 Antibody (5H9S4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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