VAMP3/Cellubrevin Antibody (4G9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97948
![Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]](https://resources.bio-techne.com/images/products/VAMP3-Cellubrevin-Antibody-4G9-Western-Blot-NBP1-97948-img0002.jpg "Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4G9
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human VAMP3/Cellubrevin (1-77aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for VAMP3/Cellubrevin Antibody (4G9) - BSA Free
Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]
Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) - BSA Free [NBP1-97948]](https://resources.bio-techne.com/images/products/VAMP3-Cellubrevin-Antibody-4G9-Flow-Cytometry-NBP1-97948-img0003.jpg "Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) - BSA Free [NBP1-97948]")
Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) - BSA Free [NBP1-97948]
Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Analysis of Cellubrevin in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)![Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]](https://resources.bio-techne.com/images/products/VAMP3-Cellubrevin-Antibody-4G9-Western-Blot-NBP1-97948-img0001.jpg "Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]")
Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]
Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Lane 1. 293T cell lysate Lane 2. Cellubrevin transfected 293T cell lysate.Applications for VAMP3/Cellubrevin Antibody (4G9) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:1000
Application Notes
Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Use in immunoprecipitation reported in scientific literature (PMID 27183589).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VAMP3/Cellubrevin
Alternate Names
CEBCellubrevin, cellubrevin, SYB3, Synaptobrevin-3, VAMP-3, vesicle-associated membrane protein 3, vesicle-associated membrane protein 3 (cellubrevin)
Gene Symbol
VAMP3
UniProt
Additional VAMP3/Cellubrevin Products
Product Documents for VAMP3/Cellubrevin Antibody (4G9) - BSA Free
Product Specific Notices for VAMP3/Cellubrevin Antibody (4G9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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