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VAP-1/AOC3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57090

Novus Biologicals, part of Bio-Techne
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NB100-57090

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-DEDPSFYSADSIY corresponding to internal region (near C-Terminus) according to NP_003725.1.

Epitope

DEDPSFYSADSIY

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VAP-1/AOC3 Antibody

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NB100-57090]

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NB100-57090]

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NB100-57090] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VAP-1/AOC3 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 55 kDa band in human adipose and lung lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 84.6 kDa band according to NP_003725.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows membranous staining in sinusoid lining cells surrounding hepatocytes.

Reviewed Applications

Read 1 review rated 4 using NB100-57090 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAP-1/AOC3

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been described and is located approximately 9-kb downstream.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Entrez Gene IDs

8639 (Human); 11754 (Mouse); 29473 (Rat)

Gene Symbol

AOC3

UniProt

Additional VAP-1/AOC3 Products

Product Documents for VAP-1/AOC3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAP-1/AOC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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