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VARS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13513

Novus Biologicals, part of Bio-Techne
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NBP2-13513
NBP2-13513-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: AVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VARS Antibody

Western Blot: VARS Antibody [NBP2-13513]

Western Blot: VARS Antibody [NBP2-13513]

Western Blot: VARS Antibody [NBP2-13513] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-13513]

Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-13513]

Immunocytochemistry/Immunofluorescence: VARS Antibody [NBP2-13513] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: VARS Antibody [NBP2-13513]

Immunohistochemistry-Paraffin: VARS Antibody [NBP2-13513]

Immunohistochemistry-Paraffin: VARS Antibody [NBP2-13513] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.

Applications for VARS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VARS

VARS - valyl-tRNA synthetase

Alternate Names

EC 6.1.1, EC 6.1.1.9, G7AVARS1, Protein G7a, valine tRNA ligase 1, cytoplasmic, Valine--tRNA ligase, ValRS, valyl-tRNA synthetase, valyl-tRNA synthetase 2, VARS2valRS

Gene Symbol

VARS1

Additional VARS Products

Product Documents for VARS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VARS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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