VARS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05970

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VARS Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human VARS fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VARS Antibody

Western Blot: VARS Antibody [NBP3-05970]

Western Blot: VARS Antibody [NBP3-05970]

Western Blot: VARS Antibody [NBP3-05970] - Lane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP3-05970]

Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP3-05970]

Immunocytochemistry/Immunofluorescence: VARS Antibody [NBP3-05970] - Staining of VARS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human VARS polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970]

Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970]

Immunohistochemistry-Paraffin: VARS Antibody [NBP3-05970] - Staining of human VARS in human brain with rabbit polyclonal antibody at 1:500 dilution.
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Applications for VARS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:2000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VARS

VARS - valyl-tRNA synthetase

Alternate Names

EC 6.1.1, EC 6.1.1.9, G7AVARS1, Protein G7a, valine tRNA ligase 1, cytoplasmic, Valine--tRNA ligase, ValRS, valyl-tRNA synthetase, valyl-tRNA synthetase 2, VARS2valRS

Gene Symbol

VARS1

Additional VARS Products

Product Documents for VARS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VARS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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