VAV2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-895

Novus Biologicals, part of Bio-Techne
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NB100-895
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VAV2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ETEAKYYRTLEDIEC corresponding to internal region according to NP_001127870.1, NP_003362.2.

Specificity

This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VAV2 Antibody

Western Blot: VAV2 Antibody [NB100-895]

Western Blot: VAV2 Antibody [NB100-895]

Western Blot: VAV2 Antibody [NB100-895] - Staining of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: VAV2 Antibody [NB100-895]

Immunohistochemistry-Paraffin: VAV2 Antibody [NB100-895]

Immunohistochemistry-Paraffin: VAV2 Antibody [NB100-895] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VAV2 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 1.5 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in 293 lysate (predicted MW of 100 kDa band according to NP_003362). IHC: Paraffin embedded Human Colon.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAV2

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Alternate Names

guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2

Entrez Gene IDs

7410 (Human); 22325 (Mouse)

Gene Symbol

VAV2

UniProt

NP_003362. (Human)

Additional VAV2 Products

Product Documents for VAV2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAV2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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