VAV2 [p Tyr142] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23268

Novus Biologicals, part of Bio-Techne
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NBP3-23268
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VAV2 Antibodies »

Product Summary for VAV2 [p Tyr142] Antibody

Immunogen

Phospho-Vav2 (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-142 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3. [UniProt# Q60992]

Modification

p Tyr142

Specificity

This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-142) peptide before affinity purification using phospho-Vav2 (Tyr-142) peptide (without carrier). The antibody detects a 100 kDa band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for VAV2 [p Tyr142] Antibody

VAV2 [p Tyr142] Antibody

Western Blot: VAV2 [p Tyr142] Antibody [NBP3-23268] -

Western Blot: VAV2 [p Tyr142] Antibody [NBP3-23268] - Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-VAV2 (Tyr-142) (lanes 3 & 4), or anti-VAV2 (Tyr-172) (lanes 5 & 6).
VAV2 [p Tyr142] Antibody

Immunocytochemistry/Immunofluorescence: VAV2 [p Tyr142] Antibody [NBP3-23268] -

Immunocytochemistry/Immunofluorescence: VAV2 [p Tyr142] Antibody [NBP3-23268] - Immunocytochemical labeling of VAV2 in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit polyclonal VAV2 (Tyr-142). The antibody was detected using goat anti-rabbit DyLight(R) 594.

Applications for VAV2 [p Tyr142] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VAV2

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav and Vav2 are members of the dbl family of guanine nucleotide exchange factors (GEF) for the rho/rac family of GTP binding proteins. Vav is expressed primarily in hematopoietic cells, while vav2 has a wider tissue distribution (2). Vav2 serves as a guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Unlike Vav1, whose expression is restricted to cells of hematopoietic origin, Vav2 is broadly expressed. Recently, Vav2 has been identified as a substrate for the epidermal growth factor (EGF) receptor. EGF receptor phosphorylates Vav2 on all three possible phosphorylation sites, Tyr-142, Tyr-159, and Tyr-172. results suggest that EGF regulates Vav2 activity basically through phosphatidylinositol 3-kinase activation, whereas tyrosine phosphorylation of Vav2 may rather be necessary for mediating protein-protein interactions (3)

Alternate Names

guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2

Gene Symbol

VAV2

Additional VAV2 Products

Product Documents for VAV2 [p Tyr142] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAV2 [p Tyr142] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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