VCAM-1/CD106 Antibody (1.4C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29413

Clone 1.4C3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.4C3

Concentration

0.2 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Localization

Cell surface

Marker

Activated Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33182)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for VCAM-1/CD106 Antibody (1.4C3)

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Detection of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.
ELISA: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

ELISA: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413]

ELISA: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Analysis (in-cell) of HDMVE cells using HRP conjugated VCAM-1 antibody. Image from verified customer review.

Applications for VCAM-1/CD106 Antibody (1.4C3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VCAM-1/CD106

VCAM1 (vascular cell adhesion molecule-1, also known as CD106 and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM1 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

UniProt

P19320

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.4C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (1.4C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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