VCAM-1/CD106 Antibody (1.G11B1) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28407

Clone 1.G11B1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Porcine

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1.G11B1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

The immunogen is a peptide made to the amino acid region VCAM1

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Specificity

Human CD106/VCAM-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VCAM-1/CD106 Antibody (1.G11B1) [PE]

Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [PE] [NBP1-28407] - Analysis of TNF alpha stimulated endothelial cell line HUV-EC-C.

Applications for VCAM-1/CD106 Antibody (1.G11B1) [PE]

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

10 uL/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and a stabilizing agent

Preservative

Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCAM-1/CD106

CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM-1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokine activated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha4beta1 (CD49d/CD29, VLA-4) and alpha4beta7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium. (1-4)

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.G11B1) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VCAM-1/CD106 Antibody (1.G11B1) [PE]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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