VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12081
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free [NBP3-12081]](https://resources.bio-techne.com/images/products/VCAM-1-CD106-Antibody-B-K9-C106-K9-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12081-img0001.jpg "Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free [NBP3-12081]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # B-K9 (C106-K9)
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing Balb/c mice against activated human umbilical vein endothelial cells (HUVEC).
Specificity
B-K9 antibody recognizes VCAM-1/CD106 (VCAM1). VCAM-1/CD106 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric - Azide and BSA Free [NBP3-12081]
Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (B-K9 (C106-K9)) - Chimeric [NBP3-12081] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of B-K9 (C106-K9) (NBP3-12081) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12081, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: VCAM-1/CD106
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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