Key Product Details

Species Reactivity

Human, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human VCAM1 (NP_001069) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

78.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free

Western Blot: VCAM-1/CD106 Antibody (OTI3H10)Azide and BSA Free [NBP2-74840]

Western Blot: VCAM-1/CD106 Antibody (OTI3H10)Azide and BSA Free [NBP2-74840]

Western Blot: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.
Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840]

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840]

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.
Immunohistochemistry: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840]

Immunohistochemistry: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840]

Immunohistochemistry: VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free [NBP2-74840] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.
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Applications for VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VCAM-1/CD106 Antibody (OTI3H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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