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VDAC1 Antibody (6B1K1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15871

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15871-100ul
NBP3-15871-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6B1K1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VDAC1 (P21796). MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VDAC1 Antibody (6B1K1)

Western Blot: VDAC1 Antibody (6B1K1) [NBP3-15871]

Western Blot: VDAC1 Antibody (6B1K1) [NBP3-15871]

Western Blot: VDAC1 Antibody (6B1K1) [NBP3-15871] - Analysis of extracts of various cell lines, using VDAC1 antibody (NBP3-15871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (6B1K1) [NBP3-15871]

Immunocytochemistry/ Immunofluorescence: VDAC1 Antibody (6B1K1) [NBP3-15871]

Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (6B1K1) [NBP3-15871] - Immunofluorescence analysis of L929 cells using VDAC1 antibody (NBP3-15871). Blue: DAPI for nuclear staining.
VDAC1 Antibody (6B1K1)

Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -

Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] - Human colon carcinoma tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for VDAC1 Antibody (6B1K1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VDAC1

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Long Name

Voltage-dependent anion-selective channel protein 1

Alternate Names

hVDAC1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC-1

Gene Symbol

VDAC1

Additional VDAC1 Products

Product Documents for VDAC1 Antibody (6B1K1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VDAC1 Antibody (6B1K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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