VDAC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-695
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Fish
Cited:
Human, Mouse, Rat, Fish, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Applications
Validated:
ELISA, Immunohistochemistry, Knockdown Validated, Simple Western, Western Blot
Cited:
Knockdown Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
VDAC1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
(Uniprot: P21796)
Reactivity Notes
A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms)
Mouse reactivity reported in scientific literature (PMID: 22728245). Rat reactivity reported in scientific literature (DOI: 10.4172/2157-7439.1000220).
Mouse reactivity reported in scientific literature (PMID: 22728245). Rat reactivity reported in scientific literature (DOI: 10.4172/2157-7439.1000220).
Localization
Mitochondria, cell membrane
Specificity
Anti-VDAC1 Antibody is directed against human VDAC1/Porin1 protein. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
Marker
Mitochondria Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for VDAC1 Antibody
Western Blot: VDAC1 Antibody [NB100-695]
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.Western Blot: VDAC1 Antibody [NB100-695]
Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).Western Blot: VDAC1 Antibody [NB100-695]
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.Applications for VDAC1 Antibody
Application
Recommended Usage
ELISA
1:15000-1:30000
Immunohistochemistry
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VDAC1
Long Name
Voltage-dependent anion-selective channel protein 1
Alternate Names
hVDAC1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC-1
Gene Symbol
VDAC1
UniProt
Additional VDAC1 Products
Product Documents for VDAC1 Antibody
Product Specific Notices for VDAC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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