VDAC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97576

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Cynomolgus Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VDAC2 Antibodies »

Product Summary for VDAC2 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human VDAC2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VDAC2 Antibody

Western Blot: VDAC2 Antibody [NBP2-97576]

Western Blot: VDAC2 Antibody [NBP2-97576]

Western Blot: VDAC2 Antibody [NBP2-97576] - Anti-VDAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HCT116 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa
Immunocytochemistry/ Immunofluorescence: VDAC2 Antibody [NBP2-97576]

Immunocytochemistry/ Immunofluorescence: VDAC2 Antibody [NBP2-97576]

Immunocytochemistry/Immunofluorescence: VDAC2 Antibody [NBP2-97576] - Immunofluorescence staining of VDAC2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human VDAC2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576]

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-97576] - Immunochemical staining of human VDAC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Applications for VDAC2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VDAC2

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. Expressed in all tissues examined. Consists mainly of membrane spanning sided beta sheets. Belongs to the eukaryotic mitochondrial porin family.

Alternate Names

FLJ23841, hVDAC2, Outer mitochondrial membrane protein porin 2, POR, VDAC-2, voltage-dependent anion channel 2, voltage-dependent anion-selective channel protein 2

Gene Symbol

VDAC2

Additional VDAC2 Products

Product Documents for VDAC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VDAC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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